Spin Break

How to Choose a Secondary Antibody

Finding the best secondary antibody to pair with your chosen primary antibody opens up an array of sometimes confusing choices. A wrong decision at this point can result in ambiguous data, incorrect data, or even no data. In this podcast, we will discuss the key considerations to keep in mind when choosing the appropriate secondary antibody.

How to Choose a Primary Antibody

Choosing a primary antibody is an important factor for the success of your experiment. This podcast will walk you through some general principles you should follow to ensure that you get the best product for your needs.

Understanding Cross-Adsorbed Antibodies

Could cross-adsorbed secondary antibodies reduce your background and improve your results? Learn about cross-adsorption and how it removes undesirable cross-reactivity in this podcast. 

Does Binding Site Matter?

Do you know where on the primary antibody your secondary antibody binds? Learn why it can impact your results and how to choose an appropriate secondary antibody.

Considerations for Multiplexing Experiments

This podcast from Bio-Rad highlights five important things to consider when you are selecting secondary antibodies for multiplexing experiments.

Tips for Choosing Secondary Antibodies

This podcast covers the main factors that impact which secondary antibodies you should use in your experiments for the best results.

The Logic Behind Biologic Antibody Drug Names

Have you noticed that therapeutic antibodies have complex and often unpronounceable names? It probably won’t surprise you to hear that there is a logic behind these names which convey information about the type of drug and its mechanism of action. In this podcast, we explain what International Non-proprietary Names are, and the logic behind their use.

The Importance of Cell Frequency

Do you know the frequency of your population of interest? Learn about the impact of cell frequency on flow cytometry experiments and how you can optimize your conditions to improve detection of rare populations in this podcast from Bio-Rad.

Challenges for the Bioanalysis of CAR-T Cells

Bio-Rad antibody expert, Michael Schwenkert, talks about the challenges for bioanalysis of CAR-T cells and how they can be addressed by the design and generation of customized anti-idiotypic antibodies.

Sample Preparation: Tips for Blood Samples

In this podcast, learn how to prepare blood samples ready for flow cytometry experiments with Bio-Rad’s top three tips.